The Blocking Solution saturates free binding sites on plastic surfaces, membranes or other protein binding surfaces. The Blocking Solution prevents nonspecific binding to surfaces. By incubating the surface with The Blocking Solution (e.g. microtiter plate or western blotting membrane), free spaces on the surface are saturated by the components of The Blocking Solution. This minimizes unwanted binding of the analyte or the detection antibody to the surface and so background can be avoided and sensitivity of the assay can be improved.
The Blocking Solution is based on chemically modified and fragmented highly purified casein.
The Blocking Solution is not comparable to other commercially available or home-made casein based solutions which are produced by just dissolving and filtration of casein.